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目的:观察重组人抵抗素(resistin)对胆管癌细胞系QBC939增殖、侵袭、转移及基质金属蛋白酶-2(MMP-2)表达的影响。方法:分别采用噻唑蓝(MTT)比色法、Matrigel侵袭实验和迁移实验检测不同浓度的抵抗素对QBC939细胞增殖、侵袭和迁移能力的影响;采用Western blot检测抵抗素对QBC939细胞内MMP-2蛋白表达的影响。结果:当抵抗素浓度高于10 ng/ml时,对QBC939细胞增殖具有明显的促进作用(P<0.01),并呈一定的剂量依赖性;经10、50、100 ng/ml抵抗素处理24 h后,QBC939细胞体外侵袭及迁移能力均较空白对照组明显增强(P<0.01);Western blot法检测抵抗素作用QBC939细胞24 h后MMP-2表达水平显著增加。结论:抵抗素对人胆管癌QBC939细胞的侵袭和转移能力有明显的促进作用,该作用机制可能与提高MMP-2表达水平有关。
Objective: To observe the effects of recombinant human resistin on the proliferation, invasion, metastasis and the expression of matrix metalloproteinase-2 (MMP-2) in human cholangiocarcinoma cell line QBC939. Methods: The effects of different concentrations of resistin on the proliferation, invasion and migration of QBC939 cells were detected by MTT colorimetric assay, Matrigel invasion assay and migration assay. Western blot was used to detect the effect of resistin on the expression of MMP-2 Effect of protein expression. Results: When the concentration of resistin was higher than 10 ng / ml, the proliferation of QBC939 cells was significantly promoted (P <0.01) in a dose-dependent manner. After treated with 10, 50 and 100 ng / ml of resistin 24 h, QBC939 cells in vitro invasion and migration were significantly enhanced compared with the blank control group (P <0.01); Western blot detection of resistin QBC939 cells after 24 h MMP-2 expression was significantly increased. Conclusion: Resistin can significantly promote the invasion and metastasis of human cholangiocarcinoma QBC939 cells, which may be related to the increase of MMP-2 expression.