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目的:观察米诺环素对糖尿病大鼠视网膜神经细胞的凋亡的影响,研究米诺环素对糖尿病视网膜神经保护作用,对米诺环素在糖尿病视网膜疾病中抑制神经细胞凋亡提供理论支持。方法:选择健康成年雄性SD大鼠30只,随机分成正常对照组、糖尿病模型组和米诺环素治疗组,每组10只。腹腔内注射链脲佐菌素(STZ)诱发大鼠糖尿病。米诺环素治疗组给予米诺环素腹腔注射(45mg/kg),共注射10d,模型组和对照组腹腔注射等体积生理盐水,于给药后8周的3组动物处死,冰浴下取其视网膜组织,随后制备视网膜石蜡切片,采用末端脱氧核糖核酸介导生物素化脱氧尿嘧啶缺口末端标记(TUNEL)法进行凋亡细胞原位标记,行视网膜神经细胞凋亡计数,对所有数据进行统计学分析。实验结果拟用均数和标准差(x±s)表示,P<0.05为差异有统计学意义。结果:相同观察时相,与阴性对照组比较,模型对照组大鼠视网膜TUNEL阳性细胞显著增多(P<0.01),在米诺环素组,大鼠视网膜TUNEL阳性细胞数比模型对照组明显减少,差异有统计学意义(P<0.01)。结论:米诺环素能有效降低糖尿病大鼠视网膜神经细胞的凋亡,对糖尿病视网膜神经细胞有保护作用。
OBJECTIVE: To observe the effect of minocycline on the apoptosis of retinal nerve cells in diabetic rats, and to study the neuroprotective effects of minocycline on diabetic retinopathy and to provide theoretical support for the inhibition of minocycline on neuronal apoptosis in diabetic retinopathy . Methods: Thirty healthy adult male Sprague-Dawley rats were randomly divided into normal control group, diabetic model group and minocycline treatment group, with 10 rats in each group. Intraperitoneal injection of streptozotocin (STZ) induced diabetic rats. The minocycline group was given intraperitoneal injection of minocycline (45mg / kg) for 10 days. The model group and the control group were injected with the same volume of normal saline intraperitoneally. Three groups of animals at 8 weeks after the administration were sacrificed, Retinal paraffin sections were obtained and retinal paraffin sections were prepared. Apoptotic cells were labeled by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), and count of retinal neurons was counted. All the data Statistical analysis. The experimental results are intended to mean and standard deviation (x ± s), P <0.05 for the difference was statistically significant. Results: Compared with the negative control group, the number of TUNEL positive cells in retina of rats in the model control group was significantly increased at the same observation phase (P <0.01). In the minocycline group, the number of TUNEL positive cells in the retina was significantly decreased compared with the model control group , The difference was statistically significant (P <0.01). Conclusion: Minocycline can effectively reduce the apoptosis of retinal neurons in diabetic rats and protect the diabetic retinal neurons.