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目的 了解强直性脊柱炎 (AS)病人外周血单个核细胞 (PBMC)基因表达谱与类风湿关节炎 (RA)及健康志愿者有无差异 ,寻找疾病发生及炎症相关的异常变化基因 ,初步探讨其变化意义。方法 以含 5 88个基因的cDNA微阵列检测 7例活动期AS、6例活动期RA病人以及 7位正常对照者PBMC基因表达谱 ,并挑选至少有 4例关节炎病人表达增多的 13个炎症反应相关的细胞因子 ,扩大研究病例数以半定量逆转录聚合酶链 (RT PCR)方法验证其表达水平。结果 cDNA微阵列检测结果显示 ,AS和RA病人基因表达谱明显异常。在正常对照组、AS和RA病人的异常高表达的基因表达率分别为 8.4 %、32 .5 %和 4 4 .8% ,两组关节炎病人与正常对照者比较P均 <0 .0 0 1;而且RA组异常表达率又明显多于AS组 (P <0 .0 12 ) ,2例活动性肺结核病人也有类似的高表达。以RT PCR法验证的13个基因的表达水平 ,发现 2 2例AS ,8例RA病人基因表达结果与cDNA微阵列检测结果一致。结论 AS病人PBMC基因表达谱明显异常 ,与RA病人也明显不同 ,13个细胞因子的高表达对AS无特异性
Objective To investigate whether gene expression profiles of peripheral blood mononuclear cells (PBMCs) and rheumatoid arthritis (RA) and healthy volunteers in patients with ankylosing spondylitis (AS) are different from those in healthy volunteers, and to find out the genes that cause abnormal changes related to the occurrence of diseases and inflammation Its meaning of change. Methods cDNA microarray with 5 88 genes was used to detect PBMC gene expression profiles in 7 active AS patients, 6 active RA patients and 7 normal controls. 13 inflammatory patients with at least 4 cases of arthritis were selected Response-related cytokines and expand the number of cases studied to verify their expression levels by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results The results of cDNA microarray showed that the gene expression profiles of AS and RA patients were significantly abnormal. In the normal control group, the gene expression rates of abnormally high expression in AS and RA patients were 8.4%, 32.5% and 44.8%, respectively, and the P values of both arthritis patients and normal controls were <0. 1, and the abnormal expression rate of RA group was significantly higher than that of AS group (P <0.12). Two active pulmonary tuberculosis patients also had similar high expression. RT-PCR method to verify the 13 gene expression levels found 22 cases of AS, 8 cases of RA gene expression results and cDNA microarray test results. Conclusions The gene expression profile of PBMC in patients with AS was significantly abnormal and distinctly different from those in RA patients. The high expression of 13 cytokines had no specific effect on AS