论文部分内容阅读
目的探讨人乳头瘤病毒(HPV)16/18感染、p53在乳腺浸润性导管癌中的表达及其相互关系。方法采用免疫组化方法和PCR技术,分别检测45例乳腺浸润性导管癌、20例乳腺纤维腺瘤及20例正常乳腺组织中HPV16/18 DNA和p53表达水平。结果乳腺癌组HPV16/18 DNA和p53蛋白的表达率分别为51.1%和46.7%,明显高于乳腺纤维腺瘤组的15.0%和10.0%和正常乳腺组的5.0%和0.0%(P<0.01);有腋淋巴结转移者的表达率明显高于无腋淋巴结转移癌者(P<0.05);随着原发肿瘤局部淋巴结远处转移(TMN)分期的升高,p53蛋白的表达率明显增加(P<0.05);HPV16/18 DNA与p53蛋白的表达有关(2χ=6.517,P<0.05)。结论 HPV感染和p53突变共同参与乳腺癌的发生和发展,HPV感染可能是促进p53突变的重要因素。
Objective To investigate the expression of human papillomavirus (HPV) 16/18 and p53 in breast invasive ductal carcinoma and its relationship. Methods Immunohistochemistry and PCR were used to detect the expression of HPV16 / 18 DNA and p53 in 45 cases of breast invasive ductal carcinoma, 20 cases of breast fibroadenoma and 20 cases of normal breast tissue. Results The positive rates of HPV16 / 18 DNA and p53 protein in breast cancer group were 51.1% and 46.7%, respectively, which were significantly higher than those in breast fibroadenoma group (15.0% and 10.0%) and those in normal breast group (5.0% and 0.0% ); The expression rate of axillary lymph node metastasis was significantly higher than those without axillary lymph node metastasis (P <0.05); With the primary tumor distant lymph node metastasis (TMN) staging, the expression of p53 protein was significantly increased (P <0.05). HPV16 / 18 DNA was associated with p53 protein expression (2χ = 6.517, P <0.05). Conclusion HPV infection and p53 mutation are involved in the occurrence and development of breast cancer. HPV infection may be an important factor in promoting the mutation of p53.