论文部分内容阅读
【背景】植物体内普遍存在一定数量的内生放线菌,对植物的生长发育具有促生作用。【目的】从番茄根内分离、筛选并鉴定出能够有效促进植物生长发育的内生放线菌,为生物菌肥的开发奠定基础。【方法】采用组织研磨培养法和放线菌分离培养基对番茄根内放线菌进行分离,利用Salkowski比色法、钼锑抗比色法和CAS平板检测法进一步筛选出具有较强促生特性的菌株,通过番茄和黄瓜苗期盆栽试验验证其促生效果。结合形态、生理生化以及16S r RNA基因序列相似性和系统发育分析,对菌株进行了鉴定。【结果】分离筛选出一株吲哚乙酸(IAA)产量达25.56 mg/L的内生放线菌NEAU-D1,能够产生铁载体并且对多种难溶性磷酸盐具有良好溶解效果,通过16S r RNA基因序列分析,该菌株属链霉菌属。番茄和黄瓜苗期盆栽试验结果表明,接种该菌株的番茄幼苗其根长、株高、植株鲜重和干重较对照分别显著增加了9%、23%、47%和92%,而接种该菌株的黄瓜幼苗根长、株高、植株鲜重和干重较对照分别显著增加了43%、47%、134%和58%。【结论】链霉菌NEAU-D1可以作为潜在的促生菌资源用于设施蔬菜多功能生物菌肥的研发。
【Background】 A certain amount of endophytic actinomycetes are commonly found in plants and have a promoting effect on the growth and development of plants. 【Objective】 Endophytic actinomycetes, which can effectively promote plant growth and development, were isolated, screened and identified from tomato root, which laid the foundation for the development of bio-fertilizer. 【Method】 Tissue culture method and actinomycete culture medium were used to separate actinomycetes in tomato root. Salkowski colorimetry, molybdenum-antimony colorimetry and CAS assay were used to further screen Characteristics of the strains, through tomato and cucumber seedlings potted test to prove its promoting effect. The strains were identified by morphological, physiological and biochemical analyzes, 16S rRNA gene sequence similarity and phylogenetic analysis. 【Result】 An endophytic actinomycete named NEAU-D1 producing 25.56 mg / L indole acetic acid (IAA) was isolated and isolated. It was able to produce iron carrier and had good solubilization effect on various insoluble phosphates. The 16S r RNA gene sequence analysis, the strain is Streptomyces. The results of pot experiment in tomato and cucumber showed that the root length, plant height, plant fresh weight and dry weight of tomato seedlings inoculated with this strain increased significantly by 9%, 23%, 47% and 92% respectively compared with the control, The root length, plant height, plant fresh weight and dry weight of the cucumber seedlings increased by 43%, 47%, 134% and 58% respectively compared with the control. 【Conclusion】 Streptomyces NEAU-D1 can be used as potential growth-promoting bacteria in the research and development of multi-functional bio-fertilizer for vegetables.