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利用软体动物大神经元探讨中枢神经系统的某些奥秘,具有很多优点:(1)胞体巨大、易于辨认,可借助显微镜直接将微电极刺入胞体或轴索内,也可将微量药物直接滴于神经元表面,进行细胞水平的研究。(2)实验条件较简单,在离体条件下的一定温度范围内,在生理溶液中(不需另外供氧),细胞可稳定地放电达数小时,有利于重复观察药物的作用。(3)可同时暴露神经元胞体及其轴索的起始部,也可同时保留该神经元所支配的内脏器官(如心、肺、肠等),便于研究神经元放电特点、神经元间的相互关系,以及神经元和所支配内脏的关系。(4)各种大神经元对于高等动物神经系统中所公认的突触传递物质(如多巴胺、
The use of large neurons in mollusks to explore some of the mysteries of the central nervous system has many advantages: (1) large cell body, easy to identify, microscopic electrodes can be directly through the cell body or axonal penetration, but also direct the trace drug On the surface of neurons, cell level studies were performed. (2) The experimental conditions are relatively simple. In a certain temperature range under ex vivo conditions, the cells can be stably discharged for several hours in a physiological solution (without additional oxygen supply), which is beneficial for the repeated observation of the effect of the drug. (3) can simultaneously expose the start of the neuronal soma and axon, but also can retain the internal organs of the neuron (such as heart, lung, intestine, etc.), to facilitate the study of neuronal discharge characteristics of interneurons Of the relationship between the neurons and the inner organs and the relationship. (4) a variety of large neurons for higher animals in the nervous system recognized synaptic transmission substances (such as dopamine,