一、材料哈士蟆油(Ovidutus Ranae):购于福州市医疗器械采购供应站,由江西中医学院范崔生教授鉴定。蟾蜍输卵管:为蟾蜍科动物黑眶蟾蜍(Bufo melanostictus Schneider)的干燥输卵管,捕捉于福州市郊,取去输卵管经60℃干燥2小时备用。二、试剂与凝胶制备按常规配制,分离胶浓度为7.5％,间隔胶浓度为2.5％。三、样品液制备分别称取哈士蟆油与蟾蜍输卵管各0.2g,置冰箱中低温固定1小时,后加饱和Na_2SO_4溶液5ml匀浆。转移至离心管中,以400rpm离心10分钟,取上清液备用。 1. Material: Ovidutus Ranae: Purchased from Fuzhou medical equipment procurement and supply station, identified by Professor Fan Cuisheng of Jiangxi University of Traditional Chinese Medicine. The fallopian tube is a dry fallopian tube of Bufo melanostictus Schneider, captured in the outskirts of Fuzhou, and the fallopian tube is dried at 60°C for 2 hours. Second, the preparation of reagents and gels according to conventional preparations, the separation gel concentration of 7.5%, spacer gel concentration of 2.5%. Third, the preparation of sample solution were weighed Huashi oyster sauce and quail oviducts each 0.2g, set in the refrigerator at low temperature for 1 hour, after adding saturated Na2SO4 solution 5ml homogenized. Transfer to a centrifuge tube, centrifuge at 400 rpm for 10 minutes, and take the supernatant for use.