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用ＮＡＤＰＨ－黄递酶（ＮＡＤＰＨ－ｄ）组织化学结合免疫细胞化学双标记方法，对大鼠部分脑区内一氧化氮合酶（ＮＯＳ）分别与５－羟色胺（５－ＨＴ）、酪氨酸羟化酶（ＴＨ）和乙酰胆碱（ＡＣｈ）等共存神经元进行了观察。结果表明在中缝背核（ＤＲ）背外侧区，绝大多数ＮＯＳ神经元与５－ＨＴ共存，但５－ＨＴ神经元仍有一半以上未表现出ＮＡＤＰＨ－ｄ阳性。在脑桥蓝斑（ＬＣ）腹内侧部存在大量ＮＯＳ神经元，其中一小部分与去甲肾上腺素（ＮＡ）能神经元（以ＴＨ为标记）共存。在中脑黑质（ＳＮ）区观察到少量ＮＯＳ神经元散在地分布干大量的多巴胺（ＤＡ）能神经元（以ＴＨ为标记）之中，此区还存在大量ＮＡＤＰＨ－ｄ阳性纤维，但没有观察到ＮＯＳ与ＤＡ共存神经元。在前脑中央隔区（ＭＳ）、Ｂｒｏｃａ斜带核水平支（ｈＤＢ）和垂直支（ｖＤＢ），９２％以上的ＮＯＳ神经元与ＡＣｈ共存，这些共存神经元约占所有胆碱能神经元的４２％。纹状体内也有不少ＮＯＳ神经元，但它们不与ＡＣｈ共存。上述ＮＯＳ分别与５－ＨＴ、ＮＡ和ＡＣｈ等经典神经递质共存的结果，为ＮＯ参与多种递质功能的调节提供了形态学基础。
NADPH-d histochemical and immunocytochemical double labeling method was used to detect the expression of nitric oxide synthase (NOS) in some brain regions of rats with 5-hydroxytryptamine (5-HT), tyrosine Coexist neurons such as hydroxylase (TH) and acetylcholine (ACh) were observed. The results showed that most of the NOS neurons co-existed with 5-HT in the dorsolateral region of the dorsal raphe nucleus (DR), but more than half of 5-HT neurons did not show positive NADPH-d. A large number of NOS neurons exist in the ventral part of the locus locus coeruleus (LC), and a small part of them coexist with noradrenaline (NA) - capable neurons (labeled with TH). A small number of NOS neurons scattered in the midbrain substantia nigra (SN) area were scattered among a large number of dopaminergic neurons (labeled with TH), which also contained a large number of NADPH-d-positive fibers but no Neurons coexisting with NOS and DA were observed. More than 92% of NOS neurons coexist with ACh in the basal forebrain (MS), BrDB (hDB) and vertical branch (vDB), and these coexisting neurons account for about 80% of all cholinergic neurons 42%. There are many NOS neurons in the striatum, but they do not coexist with ACh. The coexistence of these NOS and classical neurotransmitters such as 5-HT, NA and ACh, respectively, provided the morphological basis for NO’s involvement in the regulation of various neurotransmitter functions.