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目的研究嗜酸乳杆菌LA14菌株耗尽培养上清液(spent culture supernatant,SCS)对小鼠在体肠管运动的影响。方法昆明种小鼠50只,随机分为5组。各组分别灌胃给予SCS、活菌菌液、SCS+活菌菌液25ml/(kg.次)、硫酸阿托品注射液10 mg/(kg.次)和等容积生理盐水,每天2次,连续3 d。末次给药后1 h,各组灌胃给予印度墨汁0.2 ml/只,20 min后处死,测定小肠全长及墨汁在小肠内推进距离,计算墨汁推进率和抑制率。另取小鼠60只,随机分为6组。各组分别灌胃给予SCS、活菌菌液、SCS+活菌菌液25 ml/(kg.次)、思密达散剂1.5 g/(kg.次)或等容积生理盐水,每天2次,连续3 d。末次给药后1 h,除生理盐水组外,其余各组灌胃给予甲硫酸新斯的明注射液2 mg/kg,10 min后各组灌胃给予印度墨汁0.2 ml/只,10 min后处死,同上述方法进行测定与计算。结果在SCS组、SCS+活菌组及阿托品组,正常小鼠的墨汁推进率明显降低,与生理盐水组比较差异有非常显著性(P<0.01)。给予新斯的明造模后,小鼠的肠蠕动亢进,墨汁推进率明显升高,与生理盐水组比较差异有非常显著性(P<0.01),而给予SCS、活菌、SCS+活菌以及思密达后,由新斯的明所造成的小鼠小肠运动亢进受到明显抑制,小鼠墨汁推进率显著下降,与模型组比较差异有非常显著性(P<0.01)。结论嗜酸乳杆菌SCS能显著抑制正常小鼠的小肠推进运动;同时SCS及活菌能明显拮抗新斯的明所致的小鼠小肠运动亢进。
Objective To study the effects of spent culture supernatant (SCS) depleted in L. acidophilus LA14 on the intestinal motility in mice. Methods Fifty Kunming mice were randomly divided into five groups. Each group was given gavage SCS, viable bacteria liquid, SCS + live bacteria solution 25ml / (kg times), atropine sulfate injection 10 mg / (kg times) and the same volume of saline twice a day for 3 d. One hour after the last administration, each group was given gavage of Indian ink 0.2 ml per animal, and then sacrificed 20 minutes later. Total length of the small intestine and distance of ink in the small intestine were measured to determine the rate of ink propulsion and inhibition. Another 60 mice were randomly divided into 6 groups. Each group was given intragastric administration of SCS, viable bacterial liquid, SCS + live bacteria solution 25 ml / (kg), Smecta powder 1.5 g / (kg) or equal volume of normal saline, 2 times a day, continuous 3 d. One hour after the last administration, the rats in each group were given intragastric administration of neostigmine methionine injection 2 mg / kg at 1 h after the last administration and 0.2 ml / Executed, with the above method for determination and calculation. Results In the SCS group, SCS + live group and atropine group, the rate of ink advancement in normal mice was significantly lower than that in normal saline group (P <0.01). After giving neostigmine model, peristalsis in mice was improved and ink advancing rate was significantly higher than that in saline group (P <0.01). SCS, viable cells and SCS + Smectite, the mice caused by neostigmine induced hyperactivity of small intestine was significantly inhibited, the rate of mouse ink was significantly decreased, compared with the model group, the difference was significant (P <0.01). Conclusion Lactobacillus acidophilus SCS can significantly inhibit the small intestine propulsion of normal mice; SCS and live bacteria can significantly antagonize the neostigmine-induced mouse intestinal hyperactivity.