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的 建立C5 7BL6近交系小鼠前列腺癌原位移植瘤模型并探讨其应用价值。方法用微量注射器在C5 7BL6近交系小鼠的前列腺左右背外侧叶包膜下接种小鼠前列腺癌RM 1细胞2× 10 6个建立原位移植瘤模型 ,以皮下移植瘤模型作为对照比较两者的肿瘤转移发生情况及生存期。结果 原位模型小鼠全部发生肿瘤转移 ,其中盆腔淋巴结和肺的转移发生率分别为 ( 10 / 10 )、( 8/ 10 ) ,对照组仅有肿瘤的局部生长和浸润 ,无转移发生 ;原位模型的小鼠平均生存期为 ( 11.0±2 .4)d较对照组 ( 2 3.0± 4.7)d明显缩短 ,差异有非常显著性 ( P <0 .0 1)。结论 小鼠的前列腺癌原位移植瘤模型较好地模拟了人癌体内的自然生长状况 ,是观察转移性前列腺癌较为适宜的模型。
The establishment of C57BL6 inbred mouse prostate cancer orthotopic xenograft model and its application value. METHODS: Mice were implanted with 2×10 6 prostate cancer RM 1 cells under the capsule of the left and right dorsum of the prostate in C5 7BL6 inbred mice using a microsyringe. The subcutaneous xenograft model was used as a control. The tumor metastasis occurred and the survival period. RESULTS: All orthotopic mice developed tumor metastasis. The incidence of pelvic lymph node and lung metastasis was (10 / 10) and (8 / 10) respectively. In the control group, there was only local growth and infiltration of the tumor, and no metastasis occurred. The mean survival time of mice in the location model was (11.0 ± 2.4) days, which was significantly shorter than that in the control group (2 3.0 ± 4.7) days. The difference was significant (P <0.01). Conclusion The orthotopic xenograft model of prostate cancer in mice simulates the natural growth of human cancer. It is a suitable model for the observation of metastatic prostate cancer.