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酸性还原酮加双氧酶(ARD)催化很多原核和真核生物中的甲硫氨酸急救途径(MSP)倒数第二步。本研究鉴定了水稻白叶枯病菌Xanthomonas oryzae pv.oryzae(Xoo)中的酸性还原酮加双氧酶,命名为xard。Xoo菌株PXO99A、MAFF311018和KACC10331中的xard核苷酸序列完全相同。xard基因突变菌株在甲硫基腺苷(MTA)为唯一硫源时不能正常生长。这一结果证明Xard在MSP中起作用。xard突变体和野生型菌株PXO99A接种水稻IR24后病斑长度数据表明该基因突变对Xoo在水稻上的毒性没有影响。
Acid-reduced ketones and dioxygenases (ARDs) catalyze the second to last methionine rescue pathway (MSP) in many prokaryotes and eukaryotes. The present study identified the acid reductone dioxygenase from Xanthomonas oryzae pv. Oryzae (Xoo), designated xard. The xard nucleotide sequences in Xoo strains PXO99A, MAFF311018 and KACC10331 are identical. Xard mutant strains can not grow normally when methylthio-adenosine (MTA) is the only sulfur source. This result proves that Xard plays a role in MSP. The xard mutant and the wild-type strain PXO99A inoculated with rice IR24 after the length of diseased spots data show that the gene mutation on Xoo rice toxicity has no effect.