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应用简单重复序列区间扩增多态性(inter-simplesequencerepeats, ISSR)技术,以引种俄罗斯的西伯利亚红松(PinussibiricaDuTour)不同地理分布区的 19个种源为材料,筛选出 12个引物,获得总位点数 148条,其中多态位点数 136条,多态位点比率为 91. 89%。遗传多样性研究结果表明:种源的平均多态位点比率为 26. 48%,具有高的遗传多样性(Shannon指数(I)平均值为 0. 156 3);种源间存在一定程度的基因流动(Nm为 0. 246 1)和遗传分化(Nei指数平均值为 0. 106 8,Gst平均值为 0. 329 8);种群内的基因多样性占总群体的 67. 02%,种群间占 32. 98%。综合UPGMA聚类分析、地理变异规律及生态类型,种源划分结果为:西萨彦 -阿尔泰山地生态区种源;东西伯利亚南部高原生态区种源;西西伯利亚平原生态区种源。
Nineteen provenances from different geographic regions of Pinus sultana (Pinus sultana) introduced in Russia were selected as materials by using inter-simple sequence repeats (ISSR) technique. Twelve primers were screened to obtain total loci The number of points 148, of which 136 polymorphic loci, polymorphic locus ratio was 91.89%. The results of genetic diversity indicated that the average percentage of provenances was 26. 48%, with high genetic diversity (Shannon index (I) average value was 0.156 3). There was a certain degree of Gene flow (Nm 0. 246 1) and genetic differentiation (Nei index average 0. 106 8, Gst average 0. 329 8); within the population genetic diversity accounted for 67.02% of the total population, the population Between accounted for 32. 98%. According to UPGMA cluster analysis, geographical variation rule and ecotype, the results of provenances division are: the source of the Xishayan-Altai mountain ecological zone; the source of the plateau ecological zone in the eastern part of Siberia; the provenance of the western Siberia plain ecological zone.