本研究以巨桉嫩叶为材料,通过试剂盒法、改良CTAB法和改良SDS法,进行巨桉嫩叶总RNA提取方法的比较试验,探讨适合巨桉嫩叶总RNA提取的方法。通过超微量分光光度计、凝胶电泳和RT-PCR对总RNA提取质量进行检验,结果表明:改良CTAB和改良SDS法均能获得高质量的总RNA,条带清晰,纯度高,效果稳定,均适用于桉树叶片总RNA提取。 In this study, young leaves of E. grandis were used as materials to compare the total RNA extraction methods of E. grandis leaves with kit method, modified CTAB method and modified SDS method. Total RNA was extracted and purified by ultramicro spectrophotometer, gel electrophoresis and RT-PCR. The results showed that high quality total RNA could be obtained by both modified CTAB method and modified SDS method. The bands were clear, the purity was high, the effect was stable, All are suitable for total RNA extraction of eucalyptus leaves.