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Indo-1AM 是一种新的测定细胞内自由钙离子浓度的强荧光试剂。本文详细报导了从邻硝基酚及α-溴甲基苯甲酸酯出发经十—步反应的合成方法及其荧光性能,并提出了由 Indo-1酸o‖引入CH_3—C—OCH_2—(AM)基团的简易方法。
Indo-1AM is a new strong fluorescent reagent for measuring the concentration of free calcium in cells. In this paper, the synthesis and fluorescence properties of ortho-nitrophenol and α-bromomethylbenzoate starting from a ten-step reaction are reported in detail. The reaction of o-nitrophenol and α-bromomethylbenzoate with CH_3-C-OCH_2- (AM) group of a simple method.