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应用巢式聚合酶链反应技术，扩增急性淋巴细胞白血病患儿脑脊液细胞中Ｔ细胞受体基因Ｖδ＿２Ｄδ＿３重排片段，作为白血病细胞克隆特异性标志，检测脑脊液中微量白血病细胞。结果：２０例患儿（３６份）脑脊液中有１２例（１５份）脑脊液标本阳性，阴性对照脑脊液未见特异扩增带。提示该法诊断中枢神经系统白血病，可能是一种敏感的方法。
Nested polymerase chain reaction (PCR) was used to amplify the Vδ_2Dδ_3 rearrangement fragment of T cell receptor gene in cerebrospinal fluid of children with acute lymphoblastic leukemia and to detect leukemic cells in cerebrospinal fluid as a specific marker of leukemia cell cloning. Results: Cerebrospinal fluid was positive in 12 cases (15 samples) in cerebrospinal fluid and no specific amplification band in negative control cerebrospinal fluid in 20 cases (36 samples). Suggesting that the method of diagnosis of central nervous system leukemia, may be a sensitive method.