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为了给小麦-簇毛麦T4DL·4VS易位系在小麦育种中的利用提供依据,用T4DL.4VS易位系与来源于不同生态区的5个小麦品种郑麦9023、周9823、绵阳26、石4185、扬麦15进行杂交,杂种F1再分别与上述品种进行正反回交,研究小麦-簇毛麦T4DL·4VS易位染色体在不同小麦背景中的遗传稳定性及其在配子中的传递。原位杂交结果表明,在杂种F1花粉母细胞减数分裂中期Ⅰ,T4DL·4VS易位染色体通常可以与4D染色体配对形成棒状二价体。在不同组合的F2分离群体中,T4DL·4VS易位染色体在不同小麦遗传背景中的遗传方式不相同,T4DL·4VS易位染色体的传递受到小麦遗传背景的影响。测交结果表明,T4DL·4VS易位染色体通过雌配子和雄配子的传递率分别为50.59%(46.15%～59.1%)和24.02%(16.67%～37.75%),T4DL·4VS易位染色体通过雌配子的传递率显著高于通过雄配子的传递率。
In order to provide a basis for the utilization of the wheat-cluster-harts Triticum aestivum T4DL · 4VS translocation line in wheat breeding, T4DL.4VS translocation line was used with five wheat cultivars Zhengmai 9023, Zhou 9823, Mianyang 26, Shi 4185 and Yangmai 15 respectively, and the hybrid F1 was back-crossed with the above-mentioned varieties respectively to study the genetic stability of T4DL · 4VS translocating chromosomes in wheat and their transfer in gametes . The results of in situ hybridization showed that the chromosomes of T4DL · 4VS translocations usually can be paired with chromosome 4D to form rodlike bivalent during metaphase meiosis of hybrid F1 pollen mother cells. The genetic diversity of T4DL · 4VS translocation chromosomes in different wheat genetic backgrounds was different in F2 segregation populations with different combinations. The transmission of T4DL · 4VS translocations was influenced by the genetic background of wheat. The transcripts of T4DL · 4VS translocations showed that translocation rate of the T4DL · 4VS translocation chromosomes was 50.59% (46.15% -59.1%) and 24.02% (16.67% -37.75%), respectively. The transmissibility rate was significantly higher than that passed through male gametes.