狂犬病波及范围广，所致疾病症状严重，病死率极高，无有效治疗手段，其控制主要依赖于疫苗的接种。狂犬病毒的糖蛋白和核蛋白是有效的保护性抗原成分，常被用来制备亚单位疫苗。糖蛋白和核蛋白在大肠杆菌、酵母、痘病毒、腺病毒、杆状病毒和中国仓鼠卵巢细胞（ＣＨＯ）中均进行表达，大肠杆菌中表达出的蛋白无免疫保护作用，酵母、痘病毒、腺病毒和杆状病毒系统中表达出的蛋白在实验室和野外对动物均有一定的免疫保护作用。 Rabies spreads a wide range of diseases caused by serious symptoms, high mortality, no effective treatment, the control depends mainly on the vaccination. Rabies virus glycoprotein and nucleoprotein are effective protective antigen components, often used to prepare subunit vaccines. Glycoproteins and nucleoproteins are expressed in E. coli, yeast, poxvirus, adenovirus, baculovirus and Chinese hamster ovary cells (CHO). Proteins expressed in E. coli have no immunoprotective effect. Yeasts, poxviruses, Proteins expressed in adenovirus and baculovirus systems have some immunoprotective effects in animals both in the laboratory and in the wild.