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于1994年4－11月在东太平洋铁锰结核勘探区，随向阳红09号船，采集了39个表层沉积物、12个上覆水和7个结核样。在现场用平板法测定了异养细菌和铁、锰细菌丰度，用稀释法（MPN法）测定硫酸盐还原菌丰度。在实验室对船上带回的异养细菌和锰细菌进一步纯化分离，进行了12项生化试验，参照伯杰氏手册第八版鉴定至昆。为了探讨微生物在大洋成矿过程的作用，使用分光光度法，测定锰细菌对锰、铁离子的转化作用和不同培养温度对转化作用的影响。结果表明，在微生物丰度方面，沉积物中异养细菌的丰度在3－9500cell／g；锰、铁细菌的丰度在1×10°－103cell／g；硫酸盐还原细菌的丰度分布范围在0－4000cell／g。在上覆水和结核样中各类细菌的丰度均较低，比沉积物样低1个数量级。在细菌的菌属组成与近海区相比存在一定的差异，特别是在革兰氏阳性菌的组成上微球菌占了绝对优势。在成矿作用方面，锰细菌对锰、铁氧化还原的实验结果表明，在好氧的条件下，锰细菌使可溶性的Mn2+氧化为Mn4+,其氧化速度与环境温度存在密切关系；锰细菌对铁的氧化速度比对锰的氧化速度来得快。在厌氧的培养条件下，锰细菌能将高价铁还原为低价铁，而且使培养液的pH明显降低。
From April to November 1994, 39 surface sediments, 12 overlying water and 7 tuberculosis samples were collected in the ferromanganese nodule exploration area of the Eastern Pacific with the ship No. 09 of Xiangyang Hong. The abundance of heterotrophic bacteria, iron and manganese bacteria was measured by the plate method in the field, and the abundance of sulfate-reducing bacteria was determined by the dilution method (MPN method). Heterotrophic bacteria and manganese bacteria brought back on board were further purified and separated in the laboratory. Twelve biochemical tests were carried out. Reference is made to the eighth edition of Berger’s Handbook for identification to Kun. In order to investigate the role of microorganisms in mineralization in the ocean, spectrophotometry was used to determine the effect of manganese bacteria on the conversion of manganese and iron ions and the effect of different culture temperatures on transformation. The results showed that in the aspect of microbial abundance, the abundance of heterotrophic bacteria in the sediments ranged from 3-9500cell / g; the abundances of manganese and iron bacteria were in the range of 1 × 10 -103cell / g; the abundance distribution of sulfate-reducing bacteria Range of 0-4000cell / g. The abundance of various bacteria in overlying water and tuberculosis samples was lower than that of sediment by one order of magnitude. There is a certain difference in the composition of bacterial genus and offshore area, especially in the composition of Gram-positive bacteria Micrococcus occupies an absolute advantage. In terms of metallogenesis, the experimental results of Mn and Fe on manganese bacteria show that under aerobic conditions, manganese bacteria oxidize soluble Mn2 + to Mn4 +, and the oxidation rate is closely related to the ambient temperature. The oxidation rate is faster than the oxidation rate of manganese. Under anaerobic culture conditions, manganese bacteria can reduce high-valent iron to low-valent iron, and significantly reduce the pH of the broth.