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不锈钢(AISI 316L)是目前在医药器械中应用最为广泛的商业化材料.下一代的不锈钢智能材料将特殊功能的生物活性分子(或纳米粒子)修饰在金属表面以模拟组织功能、提高生物/细胞相容性,这是目前材料科学研究的热点领域之一.本文研究了具有微纳米多孔表面结构的316L不锈钢对抗体和生物酶分子的吸附作用,并与这些生物分子在光滑表面以及镀金表面的吸附进行了比较.研究发现不锈钢可通过简单的电化学腐蚀方法在表面产生微纳米多孔结构.微纳米孔不锈钢表面可稳定地吸附抗体或辣根过氧化物酶分子,其吸附量与喷镀金表面相当或更好.用表面活性剂(10%牛血清白蛋白(BSA)或0.2%Tween-20)洗涤不能除去吸附的蛋白.用5%Tween-20预处理金属表面,则可减少一半的抗体吸附量;但表面活性剂预处理对辣根过氧化物酶的吸附没有影响.吸附蛋白质后的金属表面湿润度大大增加;蛋白质修饰的微纳米孔不锈钢表面表现出了很好的亲水性(水接触角小于50°),指示了很好的生物相容性.而金属表面的湿润度则主要取决于蛋白质物种,并与蛋白质的吸附量正相关.吸附于不锈钢微纳米孔表面的抗体仍保持了良好的生物活性;用此种方式制备的抗CD34抗体修饰的不锈钢血管支架可以高密度并高选择性地吸附其目标细胞(如KG-1细胞).本文工作为未来制备新型的无高聚物涂层的不锈钢智能医学生物材料提供了基础.
Stainless steel (AISI 316L) is currently the most widely used commercially available material in medical devices. The next generation of stainless steel smart materials is the modification of specially functional bioactive molecules (or nanoparticles) on metal surfaces to mimic tissue function and enhance bio / Compatibility, which is one of the hottest fields in the field of materials science.We studied the adsorption of antibodies and biological enzyme molecules on 316L stainless steel with micro-nano porous surface structure, and with these biomolecules in the smooth surface and gold-plated surface Adsorption was compared.The study found that stainless steel by simple electrochemical corrosion method to produce micro-nano porous structure on the surface.Micro-nanohole stainless steel surface can be stably adsorbed antibody or horseradish peroxidase molecules, the adsorption capacity and spray gold surface Equivalent or better.The adsorbed protein can not be removed by washing with surfactant (10% bovine serum albumin (BSA) or 0.2% Tween-20) Pretreatment of the metal surface with 5% Tween-20 reduced the antibody by half However, the pretreatment with surfactant had no effect on the adsorption of horseradish peroxidase.Metal surface wettability after adsorption of protein was much Plus, the protein-modified micro-nanoporosity stainless steel surface shows good hydrophilicity (water contact angle less than 50 °), indicating good biocompatibility, while the wetting of the metal surface depends mainly on the protein species , And positively correlated with the amount of protein adsorbed.Antibody adsorbed on the surface of stainless steel micro-nanopore still maintained a good bioactivity. The anti-CD34 antibody-modified stainless steel stent prepared in this way could be adsorbed at high density and high selectivity Its target cells (such as KG-1 cells) .This work will provide a basis for the future preparation of new stainless steel smart medical biomaterials without polymer coating.