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通过盆栽实验研究一年生侧柏(Platycladus orientalis)在不同水分处理(土壤相对含水率为40%、60%、80%、100%)及重金属铅五个胁迫水平(0、300、500、1000、2000mg·kg-1)交互作用下抗氧化酶活性(POD、SOD)和丙二醛(MDA)含量的变化。结果表明:在水分和铅的交互胁迫下,侧柏幼苗叶片POD、SOD活性均表现为先升后降的趋势,不同水分胁迫条件下侧柏幼苗叶片POD、SOD活性均在铅含量为500mg·kg-1时达到最大值,在铅含量<500mg·kg-1时,不同水分胁迫条件下侧柏幼苗叶片POD、SOD活性都随着铅胁迫的增大逐渐上升,在铅含量>500mg·kg-1时,不同水分胁迫条件下侧柏幼苗叶片POD、SOD活性都随着铅胁迫的增大逐渐下降。在水分和铅的交互胁迫下,侧柏幼苗叶片MDA含量逐渐升高,在铅含量<1000mg·kg-1时,不同水分胁迫条件下侧柏幼苗叶片MDA含量变化不明显,在铅含量>1000mg·kg-1时,不同水分胁迫条件下侧柏幼苗叶片MDA含量逐渐上升。适当的交互胁迫(铅含量500mg·kg-1,60%水分处理)有利于提高侧柏的抗逆性。
Pot experiments were conducted to study the effects of five treatments (Platycladus orientalis) on five water stress treatments (relative water content 40%, 60%, 80%, 100%) and heavy metal lead (0,300,500,1000,2000mg · Kg-1), the changes of antioxidant enzyme activity (POD, SOD) and malondialdehyde (MDA) in the interaction. The results showed that the activities of POD and SOD of Platycladus orientalis showed the trend of first rising and then decreasing under the interaction of water and lead. The POD and SOD activities of Platycladus orientalis seedling leaves under different water stress were all in the lead content of 500 mg · kg-1, POD and SOD activity of Platycladus orientalis seedling leaves increased with the increase of lead stress under different water stress conditions. When the lead content was more than 500 mg · kg-1, -1, POD and SOD activities of Platycladus orientalis seedling leaves under different water stress conditions decreased with the increase of Pb stress. The MDA content of Platycladus orientalis seedling leaves gradually increased under the interaction of water and lead. The MDA content of Platycladus orientalis seedling leaves did not change significantly under different water stress conditions when the lead content was less than 1000mg · kg-1. When the lead content was above 1000mg · Kg-1, the MDA content of Platycladus orientalis seedling leaves gradually increased under different water stress conditions. Appropriate interaction stress (lead content 500mg · kg-1, 60% moisture treatment) is beneficial to improve the resistance of oriental arborvitae.