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目的:改进和建立大鼠离体肝脏灌流模型.方法:采用Krebs-Henseleit 缓冲液(pH7.4)为港灌流液基液,含2%透析48h的牛血清白蛋白组分V、20%(V:V)洗过的人红细胞和0.3%葡萄糖.灌流速度1.5ml·min~(-1)·g~(-1),温度(37±0.5)℃,灌流压力1.7～1.33pKa.测定大鼠灌流过程中胆汁分泌量、耗氧量及灌流液pH、Na~+、K~+水平,并观察肝脏外观变化和组织切片的细胞形态学,评定肝脏功能.结果:本系统灌流中大鼠的各项考察指标正常,离体肝脏的存活力可达3h.结论:该模型适用于研究药物在肝脏代谢中的相互作用及其发生机制,也可用于研究某些药物特殊的代谢动力学特征.
OBJECTIVE: To improve and establish a rat model of hepatic perfusion in vitro.METHODS: Krebs-Henseleit buffer (pH7.4) was used as a basal fluid in Hong Kong perfusion fluid, containing 2% dialyzed B 48h serum bovine serum albumin, 20% V: V) and 0.3% glucose.The perfusion rate was 1.5ml · min -1 · g -1, the temperature was 37 ± 0.5 ℃, and the perfusion pressure was 1.7 ~ 1.33pKa The bile secretion, oxygen consumption and perfusate pH, Na ~ +, K ~ + levels in rat perfusion were observed, and liver appearance changes and histological sections were observed to evaluate liver function.Results: Of the indicators of normal, the viability of isolated liver up to 3h.Conclusion: This model is suitable for the study of drug interactions in the liver metabolism and its mechanism, but also can be used to study the special pharmacokinetic characteristics of certain drugs .