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目的:探讨不同精、卵共培养时间(6h、18h)对受精卵透明带(zona pellucida,ZP)和胚胎质量的影响。方法:21个周期258枚卵子随机分成6h(A组)和18h(B组)精、卵共培养作为自身对照,受精后20h偏振光显微镜摄像,分析A、B组间正常受精卵ZP厚度(ZP thichness,ZPT)、ZP密度(ZP density,ZPD)和胚胎质量的差异。结果:A组中正常受精卵ZP内层、全层厚度和内层密度分别为8.3±1.3μm,19.0±2.6μm和2.09±0.50nm,显著低于B组(8.9±1.5μm,19.6±2.7μm,2.2±0.7nm)(P<0.05);A组发育为可移植胚胎的受精卵ZPD(2.07±0.49nm)显著低于B组(2.26±0.72nm),而发育为不可移植胚胎的受精卵外层ZPTA组(9.1±2.5μm)高于B组(7.7±1.4μm),P<0.05;受精率、可移植胚胎率及优质胚胎率A组与B组间无统计学差异,但A组移植胚胎所占比率高(76.67%vs23.33%,P<0.05)。结论:短时受精使透明带变薄,这可能有利于胚胎的发育。
Objective: To investigate the effects of different fine and co-culture time (6h, 18h) on zona pellucida (ZP) and embryo quality. Methods: 258 eggs from 21 cycles were randomly divided into 6h (A group) and 18h (B group). Egg co-culture was used as a self-control. Polarized light microscopy was performed 20 hours after fertilization. ZP thickness ZP thichness, ZPT), ZP density (ZPD) and embryo quality. Results: The inner layer, full layer thickness and inner layer density of normal zygotes in group A were 8.3 ± 1.3μm, 19.0 ± 2.6μm and 2.09 ± 0.50nm respectively, which were significantly lower than those in group B (8.9 ± 1.5μm, 19.6 ± 2.7 (P <0.05). ZPD (2.07 ± 0.49nm) was significantly lower in group A than in group B (2.26 ± 0.72nm), but fertilized embryos that developed into non-transplanted embryos The egg outer ZPTA group (9.1 ± 2.5μm) was higher than that of the B group (7.7 ± 1.4μm), P <0.05; fertilization rate, transplanting embryo rate and high quality embryo rate were not statistically different between group A and group B, but A The rate of transplanted embryos was higher (76.67% vs23.33%, P <0.05). Conclusion: Short-term fertilization thinned the zona pellucida, which may be beneficial for embryo development.