检查慢性广泛性牙周炎加重期患者16例,年龄18～49岁,牙周袋深度5.0mm～15mm。取牙周袋分泌物,用厌氧培养箱(80%氮气,10%二氧化碳,10%氢气)进行细菌培养。结果:16例患者,每例分离出细菌4～5株,共72株:①专性厌氧菌59株(81.9%),其中消化球菌16株(22.2%),梭杆菌11株(15.2%,龈紫单胞菌9株 16 patients with chronic, extensive periodontitis exacerbation were examined. The age ranged from 18 to 49 years and the periodontal pocket depth was 5.0 mm to 15 mm. Periodontal pocket secretions were taken and bacterial cultures were performed in an anaerobic incubator (80% nitrogen, 10% carbon dioxide, 10% hydrogen). Results: Among the 16 patients, there were 4 to 5 bacterial isolates in each case, with a total of 72 strains: ① 59 strains of obligate anaerobic bacteria (81.9%), of which 16 strains were cocci (22.2%), 11 strains (15.2% , 9 P. gmelinii strains