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目的:研究当归和丁酸羟基茴香醚在体外定向诱导人脂肪干细胞(hADSCs)向神经元样细胞分化的作用及对细胞的存活影响。方法:①hADSCs用诱导液(含100μL.mL-1当归注射液+DMEM/F12培养基)预诱导24 h后,用诱导液(含400μL.mL-1当归注射液+DMEM/F12培养基)进行诱导;②hADSCs用含1 mmol.L-1β-巯基乙醇(β-BME)预诱导24 h后,换用含200μmol.L-1丁酸羟基茴香醚(BHA)+2%DMSO的DMEM/F12培养基诱导。观察细胞形态变化和存活率,免疫荧光细胞化学技术检测神经元烯醇化酶(NSE)。结果:当归诱导24 h后,BHA诱导12 h后,部分hADSCs转变为神经元样细胞,当归诱导组NSE阳性率低于BHA诱导组,但存活率较高。结论:当归和丁酸羟基茴香醚可诱导hADSCs在体外分化为神经元样细胞,且当归诱导后细胞存活率较高。
Objective: To study the effect of Angelica sinensis and hydroxyanisole butyrate on the differentiation of human adipose-derived stem cells (hADSCs) into neuron-like cells in vitro and its effect on cell survival. Methods: (1) hADSCs were pre-induced with inducing fluid (containing 100μL · mL-1 Angelica Sinensis + DMEM / F12 medium) for 24 h and then induced by hindgutting fluid (containing 400μL · mL-1 angelica + DMEM / F12 medium) (2) hADSCs were pre-induced with 1 mmol.L-1β-mercaptoethanol (β-BME) for 24 h and then cultured in DMEM / F12 supplemented with 200 μmol·L-1 butyric acid hydroxyanisole (BHA) Induction. Morphological changes and survival rate were observed, and neuron enolase (NSE) was detected by immunofluorescence cytochemistry. Results: After 24 h induction of Angelica sinensis, some hADSCs were transformed into neuron-like cells 12 h after BHA induction. The positive rate of NSE in Angelica induced group was lower than that in BHA induction group, but the survival rate was higher. CONCLUSION: Angelica sinensis and hydroxyanisole butyrate can induce hADSCs to differentiate into neuron-like cells in vitro, and the cell viability after induction by Angelica sinensis is higher.