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采用聚合物掺杂法将金微粒固定在聚苯胺膜中,再通过静电吸附固定抗体,制成非标记电流型乙脑疫苗传感器。根据抗原抗体特异性结合形成的免疫复合物使电极敏感膜有效扩散面积减小,电流减少的特性,提出以嵌入金微粒的聚苯胺膜作为电子媒介体,用0.1mol/L磷酸盐缓冲液(pH7.4)为测试底液检测乙脑疫苗效价的新方法。用该传感器检测乙脑疫苗效价,线性范围6.1×10-8～4.1×10-6lgpfu/mL,相关系数r=0.994,检测限为3.0×10-8lgpfu/mL(S/N=3)。该传感器可用于生物制品的测定。
Adopt the polymer doping method to fix the gold particles in the polyaniline film, and then immobilize the antibody by the electrostatic adsorption to make the non-labeled current type JE vaccine sensor. According to the characteristics of the immunocomplex formed by specific binding of antigen-antibody to the effective diffusion area and the current decrease of the sensitive membrane of the electrode, a polyaniline membrane embedded with gold particles was proposed as an electronic mediator, and 0.1 mol / L phosphate buffer solution pH7.4) as the bottom of the test solution to test the efficacy of JE vaccine. The sensor was used to detect the titer of JE vaccine. The linear range was 6.1 × 10-8 ~ 4.1 × 10-6lgpfu / mL. The correlation coefficient was 0.994. The detection limit was 3.0 × 10-8lgpfu / mL (S / N = 3). The sensor can be used for the determination of biological products.