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用ＰＣＲ方法从ｐＰＡＩＪ．７中扩增人纤溶酶原激活剂抑制物２型（ＰＡＩ－２）基因，与ｐＰＵＣ１８重组，经限制性内切酶片段分析与核苷酸序列分析，获得全长人ＰＡＩ－２基因．ＰＡＩ－２基因与表达载体ｐＰＩＣ９重组，构建受乙醇氧化酶１基因（ＡＯＸ１）启动子与转录终止区控制的酵母表达质粒，转化ＧＳ１１５宿主菌，经表型筛选和ＰＣＲ扩增筛选阳性克隆，用甲醇诱导表达，重组ＰＡＩ－２以分泌型表达，占分泌总蛋白的３０％，具ＰＡＩ－２抗原性，与低分子量尿激酶形成了抗ＳＤＳ复合物，具抑制纤溶的活性（９１．４ＡＩＵ／ｍｌ）．对培养条件也进行了探讨．
PCR method from pPAIJ. 7 PAI-2 gene was amplified and recombined with pPUC18. The full-length human PAI-2 gene was obtained by restriction fragment analysis and nucleotide sequence analysis. The PAI-2 gene was recombined with the expression vector pPIC9 to construct a yeast expression plasmid controlled by the AOX1 promoter and transcription termination region. The recombinant plasmid was transformed into GS115 host strain. The positive clones were selected by phenotyping and PCR amplification. Methanol induced expression of recombinant PAI-2 secreted expression, accounting for 30% of total secreted protein, with PAI-2 antigen, and low molecular weight urokinase formed an anti-SDS complex with fibrinolytic activity (91.4AIU / Ml). Culture conditions were also explored.