用4个抗白粉病小麦品系构建了一个混合cDNA文库,从该文库中分离到一个新的小麦rbcL基因全长cDNA克隆.至此,已经有3个不同的小麦rbcL基因序列被报道,新rbcL基因cDNA与以前的2个序列分别有1个和3个碱基的差异.新的cDNA长1519bp 基因库查询号:AY328025 .同源性比较发现,新的小麦rbcL基因的cDNA序列与以前报告的小麦rbcL基因的cDNA序列 gi:344052 有一个碱基的差异,从而导致所编码的多肽在144位由Tyr变为Cys.进一步比较分析了在相关物种中rbcL基因的cDNA序列,结果显示,rbcL基因的编码区在不同属的物种间高度保守,并从分子水平表明小麦与大麦、新麦草、赖草、披碱草属等的亲源关系比与水稻、玉米等的近. A hybrid cDNA library was constructed from four wheat lines resistant to powdery mildew and a new full-length cDNA clone of the wheat rbcL gene was isolated from this library.Thus, three different wheat rbcL gene sequences have been reported so far. The new rbcL gene cDNA and the previous two sequences were 1 and 3 base differences.The new cDNA 1519bp gene pool accession number: AY328025.Homologous comparison found that the new wheat rbcL cDNA sequence and the previously reported wheat rbcL gene cDNA sequence gi: 344052 one base difference, resulting in the encoded polypeptide from Tyr to Cys at position 144. Further comparative analysis of the rbcL gene cDNA sequences in related species, the results show that rbcL gene The coding region is highly conserved among species belonging to different genera and at the molecular level indicates that the pro-source relationship between wheat and barley, wheat germplasm, Leymus secalis, Elymus is similar to that of rice, corn and so on.