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目的观察4,4’-二异硫氰基芪-2,2’-二磺酸(4,4’-diisothiocyanostilbene-2,2’-disulfonic acid,DIDS)对在体大鼠缺血/再灌注损伤(ischemia/reperfusion injure,I/RI)心脏的心律失常、心肌酶活性和血流动力学的影响。方法雄性SD大鼠25只随机分为5组,每组5只:假手术组、I/R组、I/R+DIDS(7 mg/kg)组、I/R+DIDS(14 mg/kg)组及I/R+DIDS(28 mg/kg)组。于再灌注即刻,经股静脉以程控微量泵给予DIDS[4 ml/(kg.h)]2 h。于缺血前、后和再灌注后,记录心脏血流动力学指标、心电图变化,再灌注后测定各组血清肌酸激酶(CK)和乳酸脱氢酶(LDH)的活性。结果DIDS可改善缺血30 min/再灌注4 h,心脏的血流动力学指标,与假手术组相比,I/R组的左室舒张末期压(LVEDP)明显升高;而左室收缩压(LVSP)、左室内压上升的最大速率(+dp/dtmax)和左室内压下降的最大速率(-dp/dtmax)明显下降(P<0.05);降低心脏心律失常积分,再灌注期间,在假手术组、I/R组、I/R+DIDS(7 mg/kg)组、I/R+DIDS(14 mg/kg)组、I/R+DIDS(28 mg/kg)组的心律失常积分值,分别是1.20±0.45,4.57±1.62,3.97±1.51,3.23±1.13和3.56±1.47,表明DIDS可明显抑制再灌注期心律失常(P<0.05),尤以I/R+DIDS(14 mg/kg)组为显著(P<0.01);减少外周血CK和LDH的活性,与I/R组相比,DIDS组LDH和CK的活性明显降低(P<0.05)。结论DIDS具有保护I/RI的作用,减少再灌注后的心律失常、抑制心肌酶释放和改善心脏的功能。
Objective To observe the effects of 4,4’-diisothiocyanostilbene-2,2’-disulfonic acid (DIDS) on ischemia / reperfusion (Ischemia / reperfusion injure, I / RI) heart arrhythmia, myocardial enzyme activity and hemodynamic effects. Methods Twenty - five male Sprague - Dawley rats were randomly divided into 5 groups: sham operation group, I / R group, I / R + DIDS (7 mg / ) Group and I / R + DIDS (28 mg / kg) group. Immediately after reperfusion, DIDS [4 ml / (kg.h)] was given via programmed femoral vein for 2 h. Cardiac hemodynamics and electrocardiographic changes were recorded before and after ischemia and after reperfusion. Serum creatine kinase (CK) and lactate dehydrogenase (LDH) activity were measured in all groups after reperfusion. Results DIDS could improve hemodynamics of the heart at 30 min of ischemia / reperfusion 4 h, LVEDP increased significantly in I / R group compared with sham-operation group, but left ventricular contraction LVSP, + dp / dtmax and -dp / dtmax were significantly decreased (P <0.05), and the cardiac arrhythmia score was decreased. During the reperfusion period, In the sham group, the heart rate was significantly higher in the I / R group, the I / R + DIDS group (7 mg / kg), the I / R + DIDS group (P <0.05), especially in I / R + DIDS (P <0.05). The results showed that DIDS could significantly inhibit the arrhythmia of reperfusion (P < 14 mg / kg) (P <0.01). Compared with I / R group, the activities of LDH and CK in DIDS group were significantly decreased (P <0.05). Conclusion DIDS has protective effects on I / RI, reduces arrhythmia after reperfusion, inhibits myocardial enzyme release and improves cardiac function.