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采用单频逆流超声技术对东北黑蜂蜂胶黄酮进行提取,研究了乙醇体积分数、温度、提取时间、液料比和占空比对黑蜂蜂胶有效成分总提取率和蜂胶中黄酮提取率的影响。在单因素试验的基础上,采用Box-Behnken中心组合试验设计对逆流脉冲超声辅助提取条件进行优化,得到最佳提取参数为:乙醇体积分数80%、提取温度51℃、提取时间16 min、液料比30︰1(mL/g)、占空比10 s/5 s,在此条件下黄酮提取率为38.72%。测定相同条件下超声提取液与未超声提液的抑菌活性,超声的蜂胶提取液和未超声的蜂胶提取液对金黄色葡萄球菌、李斯特菌、枯草芽孢杆菌均有抑菌性。
The effects of ethanol volume fraction, temperature, extraction time, liquid-to-solid ratio and duty cycle on the extraction rate of the active ingredient of black bee propolis and the extraction rate of flavonoids from propolis were studied by single-frequency counter-current ultrasonic technique. . On the basis of single-factor experiment, the Box-Behnken central composite design was used to optimize the ultrasonic assisted extraction conditions of countercurrent pulsation. The optimal extraction parameters were: ethanol volume fraction 80%, extraction temperature 51 ℃, extraction time 16 min, The material ratio is 30︰1 (mL / g) and the duty cycle is 10 s / 5 s. Under these conditions, the extraction rate of flavonoids is 38.72%. The antibacterial activity of ultrasonic extraction solution and ultrasonic extraction solution under the same conditions were determined. The ultrasonic propolis extract and non-ultrasonic propolis extract had antibacterial activity against Staphylococcus aureus, Listeria sp. And Bacillus subtilis.