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目的:研究野菊花水提液对压力负荷大鼠心肌组织胶原及信号传导的影响。方法:腹主动脉不完全结扎法(ab-dominal aortic banding,AAB)制作大鼠心肌肥厚、心室重构模型。35 d药物干预后,测量大鼠血压和心脏质量指数,取左室心肌病理切片,经HE染色测量心肌细胞横断面面积(myocardium cell cross section,MCCS);经苦味酸天狼猩红染色测量心肌组织胶原容积分数(collagen volume fraction,CVF),测量心肌血管周围胶原面积(perivascular collagen area,PVCA)和Ⅰ,Ⅲ型胶原的含量;免疫组化法测量心肌组织PKC,bFGF,P38的含量。结果:与假手术组比较,模型组动物心脏指数和心肌细胞横断面面积明显变大,血压升高,心肌组织CVF,PVCA,Ⅰ,Ⅲ型胶原生成增加,PKC,bFGF和P38表达量显著增多(P<0.05)。野菊花水提液能明显降低心脏指数和血压,缩小心肌细胞横截面面积,减少PVCA,CVF,Ⅰ,Ⅲ型胶原,降低PKC,bFGF和P38的表达(P<0.05)。结论:野菊花水提液具有减少心肌胶原沉积,抗实验性心室重构的作用,其作用机制与降低心脏的负荷,调节信号传导有关。
Objective: To study the effects of aqueous extract of Chrysanthemum on myocardial collagen and signal transduction in pressure-bearing rats. Methods: Cardiac hypertrophy and ventricular remodeling were induced by ab-dominal aortic banding (AAB). Thirty-five days after drug intervention, blood pressure and heart mass index were measured. Pathological sections of left ventricular myocardium were taken and myocardium cell cross section (MCCS) was measured by HE staining. Myocardial tissue was measured by picric acid Sirius red staining Collagen volume fraction (CVF) was measured. The content of perivascular collagen area (PVCA) and collagen type I and III were measured. The contents of PKC, bFGF and P38 in myocardium were measured by immunohistochemistry. Results: Compared with the sham-operation group, the cardiac index and myocardial cell cross-sectional area of the model group were significantly increased. The blood pressure was increased, the expression of CVF, PVCA, type I and type III collagen increased, while the expression of PKC, bFGF and P38 were significantly increased (P <0.05). The aqueous extract of wild chrysanthemum could obviously reduce the cardiac index and blood pressure, reduce the cross - sectional area of myocardial cells, reduce the expression of PVCA, CVF, collagen type Ⅰ and Ⅲ, and decrease the expressions of PKC, bFGF and P38 (P <0.05). Conclusion: The aqueous extract of wild chrysanthemum has the effect of reducing myocardial collagen deposition and anti-experimental ventricular remodeling, and its mechanism of action is related to reducing cardiac load and regulating signal transduction.