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目的:建立结肠炎散中盐酸小檗碱含量测定方法。方法:采用高效液相色谱法,用Hypersil ODS2 C_(18)色谱柱(250mm×4.6mm,5μm);流动相:乙腈-0.03mol·L~(-1)磷酸二氢钾溶液(55:45);柱温:室温;检测波长:265nm。结果:盐酸小檗碱浓度在0.04～0.2g范围内与峰面积呈良好线性关系,r=0.9996,平均回收率为100.21%(RSD为0.49%)。结论:本方法专属、重复性好,可用于结肠炎散中盐酸小檗碱的含量测定。
Objective: To establish a method for the determination of berberine hydrochloride in colitis powder. Methods: High performance liquid chromatography with Hypersil ODS2 C_(18) column (250mm×4.6mm, 5μm); mobile phase: acetonitrile-0.03mol·L -1 potassium dihydrogen phosphate solution (55:45 Column temperature: room temperature; detection wavelength: 265 nm. RESULTS: The berberine hydrochloride concentration in the range of 0.04 to 0.2 g showed a good linear relationship with the peak area, r=0.9996, and the average recovery was 100.21% (RSD was 0.49%). Conclusion: This method is specific and reproducible, and can be used for the determination of berberine hydrochloride in colitis powder.