Objective: To observe the effect of Cassia o btusifolial extract on rats of traumatic brain injury. Methods: The rats were randomly divided into 4 groups, traumatic brain injur(TBI) group,sham operation group(SHAM) group,(COB-H20 g/kg、COB-L10 g/kg) of Cassia extract groups, They were subjected to the modified Feeney's weight-drop model.sham group fake open skull window only, Cassia o btusifolial extract were given by intragastric administration and the rats were given distilled water instead as TBI and SHAM. The behavioral test was performed by Balance beam, TNF-α and IL-6 level was detected by ELASA method in serum on rats of TBI, The NSE positive cells near the region of injury was ascertainde by measuring in rats of TBI, and Western blot was used to detect the expressions of PI3K in rat brain tissues. Results: Both of Cassia extract groups and TBI traversed the beam significantly quicker than sham group at 3 time points before and after injury ( P<0.01),and both of COB groups traversed the beam significantly shorter than TBI ( P<0.05).The serum TNF-α and IL-6 content in TBI and Both of COB groups were significantly higher than sham group at 6,24,60h ( P<0.01),To compare with the TBI group, the serum TNF-αand IL-6 content in the both of COB groups were significantly decreased respectively at the time 6,24 and 60 hours, The overall level gradually decreased at 24 h, but increased slightly at 60 h. Immunohistochemical method revealed that NSE was lowered dramaticly in sham and both of COB groups( P<0.05),but it was more efficient in both of COB groups compared to TBI group ( P<0.05). the expressions of PI3 protein of the TBI group was decreased observbly than sham group( P<0.05),but COB-H group was inecreased significantly than TBI group same as sham group( P<0.05). Conclusion: Cassia o btusifolial extract can improve neurol function,increasing NSE positive cells and the expressions of PI3 protein lever.