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比较了4类(5种)不同大孔树脂对油菜籽原花青素的吸附与解吸性能,从中筛选出适用于油菜籽原花青素纯化的两种树脂,它们均属弱极性树脂。对其吸附状态及适用性进行探讨后,给出了初步的纯化工艺。结果表明,弱极性的AB-8和HPD450大孔吸附树脂对油菜籽原花青素的吸附量、吸附速率及解吸性能等均优于其它3种大孔树脂。在本实验条件下单独使用AB-8大孔树脂柱制备的油菜籽原花青素的纯度略高于HPD450大孔树脂制备的原花青素,但收率略低。经工艺优化,先采用HPD450柱,后采用AB-8柱进行分步串联纯化,则获得了更好的效果:获得原花青素干粉纯度达84%,收率为62.5%。采用50%HPD450与50%AB-8(v/v)混合树脂柱,则经一次纯化,纯度达80.3%,收率为75.2%,有一定工业价值。
The adsorption and desorption properties of four kinds of macroporous resins on rapeseed proanthocyanidins were compared. Two kinds of resins suitable for the purification of proanthocyanidins from rapeseed were screened out, which are all weak polar resins. After discussing its adsorption status and applicability, a preliminary purification process is given. The results showed that the adsorption capacity, adsorption rate and desorption capacity of pro-anthocyanin with low polarity AB-8 and HPD450 macroporous resin were better than the other three macroporous resins. Under the experimental conditions, the purity of proanthocyanidin produced by AB-8 macroporous resin column was slightly higher than that of HPD450 macroporous resin, but the yield was slightly lower. After optimization of the process, HPD450 column was firstly used and the AB-8 column was used for step-by-step purification in series to obtain better effect. The procyanidin purity was 84% and the yield was 62.5%. With a mixture of 50% HPD450 and 50% AB-8 (v / v) resin, it has a purity of 80.3% and a yield of 75.2% after a single purification, which has certain industrial value.