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基于构建的加工番茄核心种质,从3 026份自交系中选择了代表50年育种历程的615份,分别利用16个表型性状和81个(13对SSR、24对In Del、44个SNP)不同类型的多态性分子标记,对其遗传多样性进行了分析。结果表明,无论是基于表型还是基因型数据,供试自交系聚类结果基本一致,均分为两大群。其中表型聚类包括7个亚群,基因型聚类包括6个亚群。综合分析表型和基因型聚类结果,发现大多数早期(2010年以前)育成的自交系聚为一类,近年(2010年以后)育成的自交系不同程度地分布在两类中,说明近年育成的自交系遗传多样性更为丰富。在不同类型标记中,SNP在亚群中呈现更好的多态性,等位基因数、基因多样性、多态性信息量均明显高于SSR和In Del。
Based on the constructed core collection of processed tomato, 615 lines representing 50 years of breeding history were selected from 3 026 inbred lines, and 16 phenotypic traits and 81 (13 pairs of SSRs, 24 pairs of In Del, 44 SNP) different types of polymorphic molecular markers, their genetic diversity were analyzed. The results showed that the clustering results of inbred lines were basically the same whether they were based on phenotypic or genotypic data and were divided into two groups. The phenotypic cluster includes seven subgroups and the genotype cluster includes six subgroups. According to the results of phenotypic and genotypic clustering analysis, it is found that most of the inbred lines bred in early stage (before 2010) are clustered into one group. Inbred lines bred in recent years (after 2010) This indicates that the genetic diversity of bred inbred lines in recent years is more abundant. In different types of markers, SNPs showed better polymorphism in subgroups. The number of alleles, gene diversity and polymorphism information were all significantly higher than those of SSR and In Del.