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24例冠心病患者用党参口服液20ml(含生药党参20g)每日3次,治疗7天;另外10例冠心病患者每日用阿斯匹林0.5g 治疗7天作对照。经治疗,血浆 TXB_2含量在党参组从156.76±11.875pg/ml 明显减至125.01±8.855pg/ml(x±S下同),P<0.05;抑制率为15.67±5.66%,P<0.05;6-酮-PGF_(6-K)含量和抑制率均无明显变化,P>0.05。阿斯匹林组 TBX_2含量也有明显下降,P<0.05,抑制率为38.11±8.69%,P<0.001;6-K 也有较大减少,其抑制率为24.33±9.40%,P<0.05。为了揭示党参对 TXA_2和 PGI_2的作用机制,用制备的猪肺微粒体作为环氧化酶、TXA_2合成酶和 PGI_2合成酶的供酶体,用放免法分别测定了党参对由花生四烯酸或前列腺素内过氧化物合成 TXB_2及6-K 的含量。结果表明,以花生四烯酸或前列腺素内过氧化物合成的 TXB_2含量皆被党参明显抑制;在党参100mg/ml 剂量时仅明显抑制 TXB_2合成,而对6-K 生成无抑制,提示党参在此剂量时呈现 TXA_2合成酶抑制剂作用,当党参剂量为300mg/ml 时,TXA_2和PGI_2的合成酶均被抑制,P<0.001。其作用机理有待进一步研究。
24 patients with coronary heart disease were treated with Dangshen Oral Solution 20ml (containing 20g of Codonopsis pilosula) 3 times a day for 7 days; another 10 patients with coronary heart disease were treated with aspirin 0.5g daily for 7 days as a control. After treatment, plasma TXB_2 content was significantly reduced from 156.76±11.875 pg/ml in Dangshen group to 125.01±8.855 pg/ml (x±S lower), P<0.05; inhibition rate was 15.67±5.66%, P<0.05;6 There was no significant change in the content and inhibitory rate of Keto-PGF_(6-K), P>0.05. Aspirin group also had a significant decrease in TBX2 content, P<0.05, and the inhibition rate was 38.11±8.69%, P<0.001; 6-K also had a significant decrease, and the inhibition rate was 24.33±9.40%, P<0.05. In order to reveal the mechanism of Codonopsis pilosicoli on TXA_2 and PGI_2, the prepared porcine lung microsomes were used as donors for cyclooxygenase, TXA_2 synthetase, and PGI_2 synthetase, and Radix Ginseng was tested for arachidonic acid or arachidonic acid by radioimmunoassay. Prostaglandin Endoperoxide Synthesis TXB_2 and 6-K Content. The results showed that TXB_2 content synthesized by arachidonic acid or prostaglandin endoperoxide was significantly inhibited by Codonopsis pilosula; while at Codonopsis pilosula 100 mg/ml dose, TXB 2 synthesis was only inhibited obviously, but there was no inhibition on 6-K production, suggesting that Dangshen was TXA 2 synthetase inhibitors were present at this dose, and when the Dangshen dose was 300 mg/ml, both TXA 2 and PGI 2 synthase were inhibited, P<0.001. The mechanism of action needs further study.