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目的采用HPLC法测定麻花艽地上和地下部位中马钱苷酸、獐牙菜苦苷、龙胆苦苷和獐牙菜苷的含量。方法采用Eclipse XOB-C18色谱柱(150 mm×4.6 mm,5μm);流动相为甲醇-水(21∶79);流速1 mL.min-1;检测波长254 nm;柱温25℃。结果马钱苷酸、獐牙菜苦苷、龙胆苦苷和獐牙菜苷的线性范围分别为0.08～6.31μg(r=0.9997)、0.069～4.12μg(r=0.9999)、0.06～6.23μg(r=0.9998)、1.32～7.76(r=0.9999);平均回收率分别为99.7%(RSD=2.5%)、101.2%(RSD=1.9%)、102.4%(RSD=1.6%)、100.5%(RSD=2.2%)。结论所建方法测定快速、结果准确;麻花艽地上部位可作为主要的药源。
OBJECTIVE To determine the contents of rapisidine, swertiarin, gentiopicroside and swertia glycoside in the aboveground and underground parts of S. epidermis by HPLC. Methods Eclipse XOB-C18 column (150 mm × 4.6 mm, 5 μm) was used. The mobile phase was methanol-water (21:79). The flow rate was 1 mL.min-1. The detection wavelength was 254 nm. Results The linear range of horsemycin, swertiarin, gentiopicroside and swertiacetin were 0.08 ~ 6.31μg (r = 0.9997), 0.069 ~ 4.12μg (r = 0.9999), 0.06 ~ 6.23μg The average recoveries were 99.7% (RSD = 2.5%), 101.2% (RSD = 1.9%), 102.4% (RSD = 1.6%) and 100.5% (r = 0.9998) RSD = 2.2%). Conclusion The method was rapid and the results were accurate. The upper part of the twig could be used as the main drug source.