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应用 NADPH-黄递酶组织化学染色方法和巨噬细胞免疫组织化学 ABC 法,对17例静止期溃疡性结肠炎(UC)患者肠粘膜进行一氧化氮合成酶(NOS)活性测定。结果显示:静止期 UC 患者肠粘膜 NOS 阳性颗粒数(17.63±0.31个/高倍视野)及巨噬细胞数(35.85±1.02个/高倍视野)均显著高于健康对照组(分别为 P<0.05和P<0.001)表明:静止期 UC 患者肠粘膜中的 NOS 活性增高,NO 产生量增加,巨噬细胞可能是产生过量 NO 的主要来源,NO 的过量存在有可能是 UC 复发的原因之一。
Serum nitric oxide synthase (NOS) activity in intestinal mucosa of 17 patients with ulcerative colitis (UC) was measured by NADPH-diaphorase histochemical staining and macrophage immunohistochemistry ABC method. The results showed that the number of NOS positive particles (17.63 ± 0.31 high power fields) and the number of macrophages (35.85 ± 1.02 high power fields) in UC patients were significantly higher than those in healthy controls (P <0.05 and P <0.001). The results showed that the activity of NOS in the intestinal mucosa of quiescent UC patients increased and the production of NO increased. Macrophages may be the main source of excess NO production. The excessive presence of NO may be one of the causes of UC recurrence.