[目的]从甜叶菊叶片中分离纯化抗氧化内生真菌。[方法]以甜叶菊叶片为材料,从中分离纯化出内生真菌,对它们进行了液体培养,检测其发酵上清液的总抗氧化性能、清除DPPH自由基和抑制化学发光的能力。[结果]共分离获得42株内生真菌,编号为F01-F42。总抗氧化最强的为F41;清除DPPH能力最强的为F37;综合比较总抗氧化性能和清除DPPH能力,F02、F07、F20、F25、F37、F416株较突出。利用流动注射化学发光法对这6株发酵液进行检测,化学发光抑制率均大于70%,验证了它们的抗氧化性能较突出;对这6株进行了初步形态鉴定,它们的菌落颜色为白色或淡黄色,F02菌丝有隔膜,其他没有。[结论]筛选出高抗氧化活性的真菌,为将来的抗氧化研究奠定了基础。 [Objective] The research aimed to isolate and purify antioxidant endophytic fungi from Stevia leaves. [Method] The endophytic fungi were isolated and purified from the leaves of Stevia rebaudiana. Liquid culture was carried out. The total antioxidant activity of the fermentation supernatant, the scavenging DPPH free radical and the ability of inhibiting chemiluminescence were tested. [Result] Totally 42 endophytic fungi were isolated and identified as F01-F42. F41, F07, F20, F25, F37 and F416 strains were more prominent than the total antioxidant capacity and DPPH-scavenging ability. Flow injection chemiluminescence was used to detect the six fermentation broths. The chemiluminescence inhibition rates were all above 70%, which proved their anti-oxidant ability was outstanding. The preliminary morphological identification of the six strains was carried out, and their colony color was white Or light yellow, F02 hyphae membrane, the other did not. [Conclusion] The fungi with high antioxidant activity were screened, which laid the foundation for the future research of antioxidation.