体外观察s1RNA对HepG2 2.2.15细胞中乙型肝炎病毒(HBV)HBV-DNA分泌的影响。设计并合成针对HBV S区的三条siRNA,构建含上述siRNA的表达载体pSilencer ZYl、pSilencer ZY2和pSilencer ZY3,测序及酶切鉴定后用脂质体介导重组质粒转梁HepG2 2.2.15细胞,用酶免分析法对HepG2 2.2.15 细胞上清中HBV-DNA进行定量检测。结果成功构建了针对HBV S区的siRNA的表达载体,三条s1RNA 均可不同程度地抑制HepG2 2.2.15细胞上清中HBV-DNA,转染72 h抑制效果最好,分别为62%、31%、63%。说明针对HBV S区的siRNA能明显抑制HBV-DNA。 The effect of s1RNA on the HBV-DNA secretion of Hepatitis B virus (HBV) in HepG2 2.2.15 cells was observed in vitro. Three siRNAs targeting HBV S region were designed and synthesized, and the expression vectors pSilencer ZY1, pSilencer ZY2 and pSilencer ZY3 containing siRNA were constructed. After sequencing and restriction enzyme digestion, the recombinant plasmids were transfected into HepG2 2.2.15 cells with Enzyme immunoassay for quantitative detection of HBV-DNA in the supernatant of HepG2 2.2.15 cells. Results The siRNA expression vector targeting HBV S region was successfully constructed. All three s1RNAs could inhibit HBV-DNA in the supernatant of HepG2 2.2.15 cells to a certain extent. The best inhibitory effect was 72 h after transfection, which were 62%, 31% , 63%. SiRNA targeting HBV S region can significantly inhibit HBV-DNA.