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以来源于不同海拔高度的3个新疆宽刺蔷薇1年生实生苗为材料,在引种地北京测定光合作用日变化以及13:00的荧光参数、抗氧化生理指标。结果表明,来源不同海拔的宽刺蔷薇植株都在9:00以后出现净光合速率的持续降低,在上午受到气孔限制,中午则主要受非气孔限制,即PSⅡ反应中心活性的影响。各植株在13:00均受到不同程度的光抑制,与来源中、低海拔植株相比,来源高海拔的植株PSⅡ反应中心活性与热耗散能力均显著降低,即发生了更显著的光抑制效应;POD、APX酶活性以及AsA/DHA等酶类和非酶类抗氧化物质在高海拔来源植株中显著升高,以抵抗光氧化胁迫,但仍然造成了过剩的活性氧累积以及植物组织的膜脂过氧化伤害。
Three new-year seedlings of 1-year-old Rosa multiflora from different altitudes in China were used to study photosynthesis diurnal variation and fluorescence parameters and antioxidant physiological indexes at 13:00 in Beijing. The results showed that all the species of C. glauca from different altitudes had a persistent decrease in net photosynthetic rate after 9:00 and were restricted by stomatal in the morning, but non-stomatal limitation at noon, ie, the activity of PSⅡ reaction center. At 13:00, all the plants were inhibited by different degrees of light. Compared with the middle and low altitude plants, the PSⅡ reaction center activity and heat dissipation ability of plants at high altitude were significantly reduced, that is, more significant photoinhibition The activities of POD, APX and enzymes such as AsA / DHA and non-enzymatic antioxidants increased significantly in high-altitude plants to resist photooxidative stress but still resulted in the accumulation of excessive reactive oxygen species and plant tissue Membrane lipid peroxidation damage.