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目的研究Ｐ物质（ＳＰ）对人多形核白细胞（ＰＭＮ）有关方面作用及可能的意义。方法应用硝基四氮唑蓝（ＮＢＴ）还原法，荧光法和Ｇｒｉｅｓｓ反应等测定不同浓度ＳＰ单独或在细菌衍生肽类似物ＦＭＬＰ甲酯存在下对ＰＭＮ产生超氧阴离子（Ｏ－２，）过氧化氢（Ｈ２Ｏ２），一氧化氮（ＮＯ）和ＰＭＮ膜上一功能酶中性内肽酶（ＮＥＰ）活性及膜流动性的影响。结果ＳＰ（≥１０－５ｍｏｌ／Ｌ）可剌激ＰＭＮ显著产生Ｏ－２，Ｈ２Ｏ２和ＮＯ，后者被Ｌ－单甲基精氨酸（ＮＭＭＡ）所抑制；ＳＰ（１０－８～１０－４ｍｏｌ／Ｌ）能显著增加ＦＭＬＰ甲酯剌激ＰＭＮ产生Ｈ２Ｏ２，并显浓度递增依赖性；ＳＰ与ＦＭＬＰ甲酯协同可下调ＮＥＰ活性；ＳＰ能提高ＰＭＮ膜流动性。结论ＳＰ可通过ＰＭＮ介导调节炎症反应，对ＰＭＮ的杀菌功能有增强作用，ＳＰ对ＰＭＮ的这些影响可能是神经系统参与炎症和免疫调节的途径之一。
Objective To investigate the role of substance P (SP) in human polymorphonuclear leukocytes (PMN) and its possible significance. Methods Nitroblue tetrazolium (NBT) reduction, fluorescence and Griess reaction were used to determine the effect of different concentrations of SP on the production of PMN (O-2, O) alone or in the presence of the bacterially derived peptide analog FMLP methyl ester (H2O2), nitric oxide (NO) and a functional enzyme neutral endopeptidase (NEP) activity and membrane fluidity on PMN membrane. Results SP (≥10-5mol / L) stimulated PMN to produce significant O-2, H2O2 and NO, while the latter was inhibited by L-monomethyl arginine (NMMA) / L) could significantly increase the production of H2O2 by PMN in FMLP-stimulated PMN, and showed an increasing concentration dependence. SP cooperated with FMLP methyl ester to down-regulate the activity of NEP; SP increased the fluidity of PMN. Conclusion SP can mediate the inflammatory response through PMN, which can enhance the bactericidal function of PMN. These effects of SP on PMN may be one of the ways of the nervous system involved in inflammation and immune regulation.