论文部分内容阅读
目的以有效期为指标,筛选维生素K_2口服纳米乳的较优抗氧剂。方法采用高效液相色谱法测定加入抗氧剂和/或金属离子螯合剂的纳米乳中维生素K_2的含量,借助经典恒温法预测各处方纳米乳在25℃的有效期。结果未加抗氧剂的维生素K_2纳米乳的有效期为0.10年;使用单一抗氧剂或金属离子螯合剂只能将有效期提高至原来的2~4倍,而抗氧剂与金属离子螯合剂联用可将制剂的有效期提高至原来的20倍以上,其中维生素C棕榈酸酯与Ca Na2EDTA联用,可使纳米乳的有效期提高24倍,可达2.36 a。结论在处方中加入抗氧剂与金属离子螯合剂后可显著提高纳米乳的有效期,其中维生素C棕榈酸酯与Ca Na2EDTA联用的抗氧化效果最佳。
Objective To expire the validity of the index screening of vitamin K_2 oral nanoemulsion better antioxidants. Methods The content of vitamin K 2 in nanoemulsion with antioxidants and / or metal chelators was determined by high performance liquid chromatography (HPLC), and the predictive value of the nanoemulsion at 25 ℃ was obtained by classical thermostat method. Results without antioxidant vitamin K_2 nanoemulsion is valid for 0.1 years; the use of a single antioxidant or metal ion chelating agent can only be effective period increased to 2 to 4 times the original, and antioxidants and metal ion chelator With the formulation can be increased to more than 20 times the original validity, of which vitamin C palmitate and Ca Na2EDTA combined, nanoemulsions can be increased by 24 times, up to 2.36 a. Conclusion Antioxidant and metal ion sequestrant could increase the expiration date of the nanoemulsion in the prescription, and the combination of vitamin C palmitate and Ca Na2EDTA had the best antioxidation effect.