目的探讨 c-myc 小干扰 RNA 对急性粒细胞白血病细胞株 HL-60增殖、凋亡、基因、蛋白表达的影响。方法利用 T7 RNA 聚合酶和双链 DNA 模板采取体外转录法合成小干扰 RNA。合成的 c-myc 小干扰 RNA,经 Lipo-fectamine 2000脂质体转染入 HL-60细胞,观察形态学变化,应用四唑盐法(MTT)绘制细胞生长曲线,细胞集落培养 Objective To investigate the effects of c-myc siRNA on the proliferation, apoptosis, gene and protein expression of acute myeloid leukemia HL-60 cells. Methods T7 RNA polymerase and double stranded DNA template were used to synthesize small interfering RNA by in vitro transcription. The small interfering RNA of c-myc was transfected into HL-60 cells with Lipofectamine 2000. Morphological changes were observed. The cell growth curve was drawn by tetrazolium salt (MTT)