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目的通过观察枸杞多糖对去卵巢大鼠血脂、肝脏形态及肝脏中P-GSK-3β和PPARγ蛋白表达的影响,探究枸杞多糖对去卵巢大鼠脂代谢的干预作用及机制。方法 30只SPF级健康雌性SD大鼠随机分为假手术组、去卵巢组、补佳乐组、枸杞多糖高剂量组及枸杞多糖低剂量组(每组6只)。全自动生化分析仪检测大鼠血脂水平,HE染色检测各组大鼠肝脏组织形态学变化,Western Blot方法检测肝脏中P-GSK-3β和PPARγ蛋白表达。结果血脂检测发现,与假手术组相比,去卵巢组TC、LDL-C水平显著升高(P<0.01),HDL-C水平显著降低(P<0.01),与去卵巢组相比,补佳乐组与枸杞多糖高剂量组TC、LDL-C水平显著降低(P<0.01),枸杞多糖低剂量组LDL-C水平降低(P<0.05)。HE染色发现,去卵巢组可见散在的脂肪空泡,枸杞多糖高剂量组脂肪空泡减少。Western Blot结果显示,与假手术组相比,去卵巢组P-GSK-3β与PPARγ表达水平显著下调(P<0.01);与去卵巢组相比,补佳乐组与枸杞多糖高剂量组P-GSK-3β与PPARγ表达水平升高(P<0.05或P<0.01)。结论高剂量枸杞多糖可能通过上调肝脏中p-GSK-3β与PPARγ表达改善脂代谢紊乱。
OBJECTIVE: To observe the effects of lycium barbarum polysaccharides (LBP) on lipid and liver morphology and the expression of P-GSK-3β and PPARγ in ovariectomized rats, and explore the intervention mechanism and mechanism of LBP on lipid metabolism in ovariectomized rats. Methods Thirty SPF healthy female SD rats were randomly divided into sham-operation group, ovariectomized group, high-yield group, high-LBP group and low-LBP group (6 in each group). Blood lipid levels were detected by automatic biochemical analyzer. The morphological changes of liver were detected by HE staining. The protein expression of P-GSK-3β and PPARγ in liver were detected by Western Blot. Results Compared with sham operation group, TC and LDL-C levels in ovariectomized group were significantly increased (P <0.01) and HDL-C levels were significantly decreased (P <0.01) The levels of TC and LDL-C in Jiale group and LBP high-dose group were significantly decreased (P <0.01), LDL-C level was lower in LBP low dose group (P <0.05). Hematoxylin and eosin (HE) staining showed scattered vacuolar vacuoles in the ovariectomized group and reduced fat vacuoles in the high-dose LBP group. Western Blot results showed that compared with sham operation group, the expression of P-GSK-3β and PPARγ in ovariectomized group was significantly decreased (P <0.01); Compared with ovariectomized group, -GSK-3β and PPARγ expression (P <0.05 or P <0.01). Conclusion High-dose LBP may improve lipid metabolism disorder by up-regulating the expression of p-GSK-3β and PPARγ in the liver.