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Bone marrow mesenchymal stem cells were isolated from C57BL mice,transfected with the cytosine deaminase(CD) gene using a lentivirus vector and co-cultured with C6 glioma cells to verify anti-tumor effects of bone marrow mesenchymal stem cells carrying CD genes.C57MSC-CD/eGFP cells converted 5-fluorocytosine to 5-fluorouracil and exhibited significant inhibition of proliferation and apoptosis in C6 glioma cells.C57MSC-CD/eGFP cells were then implanted into rat models of brain C6 glioma.Rats were also intraperitoneally injected with 5-fluorocytosine after 7 days.MSC-CD/eGFP cells were irregularly distributed at the margin of the glioma,as well as encased and reduced the volume of the glioma.CD-transfected bone marrow mesenchymal stem cells inhibit the in vivo growth and in vitro proliferation of glioma.
Bone marrow mesenchymal stem cells were isolated from C57BL mice, transfected with the cytosine deaminase (CD) gene using a lentivirus vector and co-cultured with C6 glioma cells to verify anti-tumor effects of bone marrow mesenchymal stem cells carrying CD genes. C57MSC- CD / eGFP cells converted 5-fluorocytosine to 5-fluorouracil and exhibited significant inhibition of proliferation and apoptosis in C6 glioma cells. C57MSC-CD / eGFP cells were then implanted into rat models of brain C6 glioma.Rats were also intraperitoneally injected with 5- fluorocytosine after 7 days. MSC-CD / eGFP cells were irregularly distributed at the margin of the glioma, as well as encased and reduced the volume of the glioma. CD-transfected bone marrow mesenchymal stem cells inhibit the in vivo growth and in vitro proliferation of glioma.