论文部分内容阅读
目的考察苦碟子注射液对大鼠脑缺血再灌注损伤的保护作用,并从炎症角度探讨其保护机制。方法改良线栓法建立大鼠大脑中动脉栓塞(MCAO)模型,激光多普勒血流仪(LDF)监测大鼠脑血流变化。雄性SD大鼠随机分为假手术、模型组及苦碟子注射液高、低剂量组。缺血2 h再灌注24 h后进行神经功能缺损评分;断头取闹,称脑湿质量,计算脑指数;TTC染色法检测脑梗死面积;HE染色观察脑组织病理形态;并取血、脑组织,Elisa试剂盒检测炎症因子表达,Western Blotting技术检测TLR-4、NF-κB蛋白表达。结果与假手术组比较,模型组神经功能缺损较严重(P<0.01),脑指数和脑梗死面积也显著升高;给予苦碟子注射液后能改善神经功能缺损症状(P<0.01),显著降低模型组的脑指数和脑梗死面积,减少神经元坏死,同时苦碟子注射液还可显著降低脑匀浆和血清TNF-α水平(P<0.05),增加局部脑组织IL-10水平(P<0.05)。Western Blotting结果显示,苦碟子注射液可降低TLR-4、NF-κB蛋白的表达(P<0.05)。结论苦碟子注射液对大鼠脑缺血再灌注损伤有明确的保护作用,具有降低脑缺血再灌注后TNF-α,并升高IL-10水平的作用,其作用机制可能与其下调TLR-4/NF-κB信号通路有关。
Objective To investigate the protective effect of Kudiezi Injection on cerebral ischemia-reperfusion injury in rats and its protective mechanism from the perspective of inflammation. Methods The rat model of middle cerebral artery occlusion (MCAO) was established by the method of modified thread and the cerebral blood flow was monitored by laser Doppler flowmetry (LDF). Male Sprague-Dawley rats were randomly divided into sham operation, model group and Kudiezi injection high and low dose groups. Neurological deficit scores were measured at 2 h after reperfusion for 24 h after reperfusion. The animals were sacrificed by decapitation, brain wet mass was calculated and brain index was calculated. The infarct size was detected by TTC staining. The pathological changes of brain were observed by HE staining. Elisa kit was used to detect the expression of inflammatory cytokines. Western Blotting was used to detect the expression of TLR-4 and NF-κB. Results Compared with the sham-operation group, the neurological deficit in the model group was more severe (P <0.01), and the area of cerebral index and cerebral infarction were also significantly increased. After KDZ injection, the symptoms of neurological deficits were improved (P <0.01) Decreased brain index and cerebral infarction area and decreased neuronal necrosis in the model group. At the same time Kudiezi injection can significantly reduce brain homogenate and serum TNF-α levels (P <0.05), increase the level of IL-10 in brain tissue (P <0.05). Western Blotting results show that kudzu injection can reduce the expression of TLR-4, NF-κB protein (P <0.05). Conclusion Kudiezi injection has a definite protective effect on cerebral ischemia-reperfusion injury in rats, which can decrease the level of TNF-α and increase the level of IL-10 after cerebral ischemia / reperfusion, and its mechanism may be related to the decrease of TLR- 4 / NF-κB signaling pathway.