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目的观察骨软骨瘤组织Sox9、Osterix及PTEN蛋白表达水平。方法选择2010年1月—2015年12月收治的骨软骨瘤患者30例作为观察组,选择同期行髋脱位矫正手术的患者30例作为对照组,取观察组软骨瘤组织和对照组软骨组织,用免疫组化SP法测定Sox9、Osterix及PTEN蛋白表达水平。计量资料比较采用t检验,计数资料比较采用χ~2检验,P<0.05为差异有统计学意义。结果观察组Sox9、Osterix平均灰度值为[(158.6±11.5)、(171.6±21.5)]高于对照组[(105.2±13.6)、(118.6±16.5)],且Sox9与Osterix表达水平呈正相关(r=0.531),差异有统计学意义(均P<0.05);观察组PTEN蛋白阳性表达23例(76.7%)低于对照组29例(96.7%),差异有统计学意义(P<0.05)。结论骨软骨瘤组织Sox9、Osterix表达升高,PTEN蛋白表达水平降低,Sox9、Osterix及PTEN蛋白表达的异常可能与骨软骨瘤形成具有密切关系。
Objective To observe the expression of Sox9, Osterix and PTEN protein in osteochondroma. Methods Thirty patients with osteochondroma who were treated from January 2010 to December 2015 were selected as the observation group and 30 patients undergoing hip replacement surgery during the same period were selected as the control group.The cartilage tissues of the observation group and the control group were selected, The expression of Sox9, Osterix and PTEN protein was detected by immunohistochemical SP method. Measurement data were compared using t test, count data were compared using χ ~ 2 test, P <0.05 for the difference was statistically significant. Results The average gray value of Sox9 and Osterix in the observation group was (158.6 ± 11.5) and (171.6 ± 21.5), respectively, higher than that in the control group (105.2 ± 13.6 and 118.6 ± 16.5), respectively (r = 0.531), the difference was statistically significant (all P <0.05). The positive expression of PTEN protein in observation group was lower than that in control group (76.7% ). Conclusion The expression of Sox9 and Osterix in osteochondroma increased and the expression of PTEN decreased. The abnormal expression of Sox9, Osterix and PTEN may be closely related to the formation of osteochondroma.