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两个组合(中86-44×YD1785、中156×3-66)的种间杂种(O.sativa×O.officinalis)F1植株,完全不育。以栽培稻为轮回亲本进行回交,结合胚拯救技术,获得两组合回交一代(BC1F1)植株。两组合回交一代(BC1F1)群体中分别出现6个和7个类型的植株,完全不育。通过继续回交获得的组合中86-44×YD1785回交二代(BC2F1)植株中,出现了4个类型的植株,株型似栽培稻,大部分植株可育。经自交获得株型好,结实正常,抗性强的中间材料。结果表明:(1)栽野杂种回交世代出现疯狂分离,药用野生稻遗传上存在较大异质性;(2)通过连续两次回交,结合胚拯救技术,可改变杂种野生性状,获得具有药用野生稻优良基因的中间材料,如75-2、79-1、79-11三个株系;(3)证实胚拯救技术在种间杂交转移药用野生稻有利基因中是一条行之有效的途径。
F1 plants of two combinations (86-44 × YD1785, 156 × 3-66) were completely sterile. Backcrossing cultivated rice as the recurrent parent, and combining the embryo rescue technology, two combinations of backcross generation (BC1F1) plants were obtained. The two combinations of backcross generation (BC1F1) population appeared in 6 and 7 types of plants, completely sterile. Of the 86-44 × YD1785 backcross second generation (BC2F1) plants obtained by continued backcrossing, four types of plants appeared, the plant type resembling cultivated rice, and most of the plants were fertile. Obtained by self-plant type is good, strong normal, strong resistance of the intermediate material. The results showed that: (1) the backcrossing generation of wild-type hybrids appeared crazy separation and the genetic heterogeneity existed in medicinal wild rice; (2) The wild-type hybrids could be changed by two successive backcrossing and embryo rescue techniques Intermediate materials with excellent genes of medicinal wild rice, such as three lines 75-2, 79-1 and 79-11; (3) Confirming that embryo rescue technology is a line in interspecific hybridization and transfer of wild rice The effective way.