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为了深入研究免疫－神经－内分泌网络，用免疫细胞化学技术，检测了大鼠海马和下丘脑白细胞介素－２受体（ＩＬ－２Ｒ）的分布和体外培养海马神经细胞中ＩＬ－２Ｒ的表达，以及糖皮质激素处理对体内、体外实验中ＩＬ－２Ｒ表达的影响。结果表明：高度密集的ＩＬ－２Ｒ免疫反应神经元分布在下丘脑诸核，特别是弓状核、腹内侧核、背内侧核、室旁核。中等密度的ＩＬ－２Ｒ免疫反应神经元分布在海马锥体细胞层和齿状回颗粒细胞层和多形细胞层。在培养的海马神经元中，有部分神经元呈ＩＬ－２Ｒ免疫反应阳性。大鼠经地塞米松处理（５ｍｇ／ｋｇ／ｄ，ｉｐ）３ｄ后，上述脑区ＩＬ－２Ｒ表达明显降低。下丘脑阳性神经元减少，海马和齿状回阳性细胞几乎消失。培养的海马神经元经地塞米松（５０ｍｇ／Ｌ）温育２４ｈ后，ＩＬ－２Ｒ免疫反应细胞显著减少。结果提示：糖皮质激素可通过调节ＩＬ－２Ｒ在上述区域的表达而影响神经细胞的功能。本研究为免疫－神经－内分泌网络学说提供了部分直接的形态学证据。
In order to further study the immune-neuroendocrine network, the distribution of interleukin-2 receptor (IL-2R) in the hippocampus and hypothalamus of rats and the expression of IL-2R in cultured hippocampal neurons were detected by immunocytochemistry , As well as glucocorticoid treatment on in vivo and in vitro experiments IL-2R expression. The results showed that highly dense IL-2R immunoreactive neurons were distributed in hypothalamus nuclei, especially arcuate nucleus, ventral medial nucleus, dorsal medial nucleus and paraventricular nucleus. Middle-density IL-2R immunoreactive neurons are distributed in the hippocampal pyramidal cell layer and the dentate gyrus granulocyte and pleomorphic cell layers. In cultured hippocampal neurons, some neurons showed IL-2R immunoreactivity. After dexamethasone treatment (5 mg / kg / d, ip) for 3 days, the expression of IL-2R in the brain regions was significantly decreased. Hypothalamus-positive neurons decreased, hippocampal and dentate gyrus-positive cells almost disappeared. Cultured hippocampal neurons dexamethasone (50mg / L) after incubation 24h, IL-2R immunoreactive cells was significantly reduced. The results suggest that glucocorticoids affect the function of nerve cells by regulating the expression of IL-2R in these areas. This study provides some direct morphological evidence for the immune-neuroendocrine network theory.