目的:运用高效液相色谱法测定木鳖子中3-0-6’-0-甲基-β-D葡萄糖醛酸丝石竹苷的含量。方法:ECONO-SPHERE C_(18)色谱柱,以甲醇-水(80:20)为流动相,流速0.8mL·min~(-1),检测波长210nm。结果:3-0-6’-0-甲基-β-D-葡萄糖醛酸丝石竹苷线性范围0.612～3.672μg,r=0.999 9;平均加样回收率98.8％;RSD=0.36％(n=5)。结论:该法灵敏、准确,可作为衡量木鳖子质量的依据。 Objective: To determine the content of 3-0-6’-O-methyl-β-D-glucuronic acid stevioside in Hibiscus wilsonii by high performance liquid chromatography. METHODS: ECONO-SPHERE C_(18) column with methanol-water (80:20) as mobile phase, flow rate of 0.8 mL·min -1, detection wavelength at 210 nm. RESULTS: The linear range of 3-0-6’-O-methyl-β-D-glucuronic acid stevioside was 0.612～3.672μg, r=0.999 9; the average recovery was 98.8%; RSD was 0.36% (n =5). Conclusion: This method is sensitive and accurate, and it can be used as a basis for measuring the quality of Dendrobium.